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Specimen: 5 mL blood in plain, lithium heparin or EDTA tube.

Method: Extraction and conversion of methaemalbumin to haemochromogen; spectroscopy.

Reference Interval: Negative.

Application: Investigation of possible intravascular haemolysis.

Interpretation: Once haptoglobins have been depleted by intravascular haemolysis, free haem binds to albumin to form methaemalbumin, which is detected by the Schummís test.

Reference: Dacie JV and Lewis SM. Practical Haematology. 8th ed. Churchill Livingstone 1995.

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